Sucrose is thus a non-reducing sugar which does not react with Benedict's reagent. Sucrose indirectly produces a positive result with Benedict's reagent if heated with dilute hydrochloric acid prior to the test, although after this treatment it is no longer sucrose.
The acidic conditions and heat break the glycosidic bond in sucrose through hydrolysis. The products of sucrose decomposition are glucose and fructose, both of which can be detected by Benedict's reagent, as described above. Starches do not react or react very poorly with Benedict's reagent, due to the relatively small number of reducing sugar moieties, which occur only at the ends of carbohydrate chains. Inositol myo-inositol is another carbohydrate which produces a negative test.
Benedict's reagent can be used to test for the presence of glucose in urine. Glucose found to be present in urine is an indication of diabetes mellitus. Glucose is called reducing sugar because it is capable of transferring hydrogen to other compounds. Such a process is known as reduction or redox reaction. Your email address will not be published. Chemistry Learner It's all about Chemistry.
Excess Reagent. Metathesis Reaction. E2 Reaction. Keep logged in. Cookies deactivated. To use all functions of this page, please activate cookies in your browser. Login Register. Additional recommended knowledge. Topics A-Z. All topics. To top. About chemeurope. Colorimetry-Software Day Free Trial. I performed this particular experiment according to the outlined steps. The result l had was a dark brown color.
What might be the cause of this result? So 2 ml would be 40 drops. What should the protocol say? There is one major problem in this writing…. Hydrogens are protons…often, having given away an electron, they acquire a positive charge. It is also quite difficult to clean any glassware after that mistake.
Hydrogens are not electrons, they are protons and often have a positive charge. Is it possible that the sugars are reducing sugars because they accept hydrogens instead of give them up?
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